Cloning and Sequencing of the ompA Gene of Enterobacter sakazakii and Development of an ompA -Targeted PCR for Rapid Detection of Enterobacter sakazakii in Infant Formula

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Cloning and sequencing of the ompA gene of Enterobacter sakazakii and development of an ompA-targeted PCR for rapid detection of Enterobacter sakazakii in infant formula.

Enterobacter sakazakii is an emerging, infant formula-borne pathogen that causes severe meningitis, meningoencephalitis, sepsis, and necrotizing enterocolitis in neonates and infants, with a high fatality rate. Traditional detection methods take up to 7 days to identify E. sakazakii. The outer membrane protein A gene (ompA), along with its flanking sequences from E. sakazakii (ATCC 51329), was ...

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Enterobacter sakazakii: an emerging pathogen in powdered infant formula.

Enterobacter sakazakii represents a significant risk to the health of neonates. This bacterium is an emerging opportunistic pathogen that is associated with rare but life-threatening cases of meningitis, necrotizing enterocolitis, and sepsis in premature and full-term infants. Infants aged <28 days are considered to be most at risk. Feeding with powdered infant formula (PIF) has been epidemiolo...

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development and implementation of an optimized control strategy for induction machine in an electric vehicle

in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...

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Powder infant formula milk contaminated with Enterobacter sakazakii.

To clarify the route and source of Enterobacter sakazakii infection in a basic study, we analyzed powder infant formula milk (PIF), which may be an important source of infantile infection, regarding contamination with Enterobacteriaceae including this type of bacteria, and conducted drug sensitivity tests with various antimicrobial agents. Enterobacteriaceae was isolated 36 (24.2%) of 149 PIF s...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2006

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.72.4.2539-2546.2006